IKK alpha + IKK beta Recombinant Rabbit Monoclonal Antibody [SN63-02]
cat.: ET1611-23
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | SN63-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 87 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IKK beta aa 450-652 / 756. |
| Positive control: | Hela cell lysate, A431 cell lysate, Daudi cell lysate, Hela, B16F1, C2C12. |
| Subcellular location: | Cytoplasm, Nucleus, Membrane raft. |
| Recommended Dilutions:
WB IF-Cell IP |
1:500-1:1,000 1:100-1:500 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: O14920 Human | O15111 Human | O88351 Mouse | Q60680 Mouse | Q9QY78 Rat |
| Alternative names: | CHUK Conserved helix loop helix ubiquitous kinase I kappa B kinase 1 I kappa B kinase 2 I Kappa B kinase alpha I Kappa B kinase beta IkB kinase alpha subunit IkBKA IkBKB IKK a kinase IKK alpha IKK beta IKK1 IKK2 IKKA IKKB IMD15 Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta Inhibitor of nuclear factor kappa-B kinase subunit alpha Inhibitor of nuclear factor kappa-B kinase subunit beta NFKBIKA NFKBIKB Nuclear factor NF kappa B inhibitor kinase beta Nuclear factor NFkappaB inhibitor kinase alpha TCF 16 TCF16 Transcription factor 16 |
Images
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Fig1:
Western blot analysis of IKK alpha + IKK beta on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: A431 cell lysate Lane 3: Daudi cell lysate |
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Fig2: ICC staining of IKK alpha + IKK beta in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of IKK alpha + IKK beta in B16F1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of IKK alpha + IKK beta in C2C12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.