Anti-Phospho-c-Myc(T58) antibody [SN60-01]
cat.: ET1611-24
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC/IF, FC, IHC-P
Clonality: Monoclonal
Clone number: SN60-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 50 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr58 of human Myc.
Positive control: Hela, SKOV-3, human kidney tissue, MCF-7.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  FC
  ICC/IF
  IHC-P

1:1,000
1:50-1:100
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P01106 Human
Alternative names: Avian myelocytomatosis viral oncogene homolog bHLHe39 c Myc Class E basic helix-loop-helix protein 39 MRTL Myc Myc protein Myc proto oncogene protein Myc proto-oncogene protein myc related translation/localization regulatory factor MYC_HUMAN Myc2 MYCC Niard Nird Proto-oncogene c-Myc Transcription factor p64 v myc avian myelocytomatosis viral oncogene homolog v myc myelocytomatosis viral oncogene homolog
Images
ET1611-24_1.jpg Fig1: ICC staining of Phospho-c-Myc(T58) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-24, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-24_2.jpg Fig2: ICC staining of Phospho-c-Myc(T58) in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-24, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-24_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-c-Myc(T58) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-24, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-24_4.jpg Fig4: Flow cytometric analysis of Phospho-c-Myc(T58) was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-24, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.