Phospholipase C gamma 1 Recombinant Rabbit Monoclonal Antibody [SN06-10]
cat.: ET1611-25
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | SN06-10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 149 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Phospholipase C gamma 1 aa 690-852 / 1,290. |
| Positive control: | HeLa cell lysate, PC-12 cell lysate, mouse lung tissue lysate, mouse brain tissue lysate, rat lung tissue lysate, rat brain tissue lysate, NIH/3T3 cell lysate, SHG-44, MCF-7. |
| Subcellular location: | Cell projection. |
| Recommended Dilutions:
WB IF-Cell IP |
1:1,000 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P19174 Human | Q62077 Mouse | P10686 Rat |
| Alternative names: | 1 phosphatidyl D myo inositol 4 5 bisphosphate 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 Inositoltrisphosphohydrolase Monophosphatidylinositol phosphodiesterase NCKAP3 Phosphatidylinositol phospholipase C Phosphoinositidase C Phosphoinositide phospholipase C Phosphoinositide phospholipase C-gamma-1 Phospholipase C 148 Phospholipase C gamma 1 Phospholipase C-gamma-1 Phospholipase C-II PLC gamma 1 PLC II PLC-148 PLC-gamma-1 PLC-II PLC1 PLC148 Plcg1 PLCG1_HUMAN PLCgamma1 |
Images
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Fig1:
Western blot analysis of Phospholipase C gamma 1 on different lysates with Rabbit anti-Phospholipase C gamma 1 antibody (ET1611-25) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: PC-12 cell lysate (20 µg/Lane) Lane 3: Mouse lung tissue lysate (40 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: Rat lung tissue lysate (40 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Lane 7: NIH/3T3 cell lysate (20 µg/Lane) Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-25) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Phospholipase C gamma 1 in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-25, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of Phospholipase C gamma 1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-25, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.