Phospholipase C gamma 1 Recombinant Rabbit Monoclonal Antibody [SN06-10]
cat.: ET1611-25
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IP, IHC-P
Clonality: Monoclonal
Clone number: SN06-10
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 149 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Phospholipase C gamma 1 aa 690-852 / 1,290.
Positive control: HeLa cell lysate, PC-12 cell lysate, mouse lung tissue lysate, mouse brain tissue lysate, rat lung tissue lysate, rat brain tissue lysate, NIH/3T3 cell lysate, SHG-44, MCF-7.
Subcellular location: Cell projection.
Recommended Dilutions:
  WB
  IF-Cell
  IP
  IHC-P
1:1,000
1:50-1:200
Use at an assay dependent concentration.
1:200
Uniprot #: SwissProt: P19174 Human | Q62077 Mouse | P10686 Rat
Alternative names: 1 phosphatidyl D myo inositol 4 5 bisphosphate 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 Inositoltrisphosphohydrolase Monophosphatidylinositol phosphodiesterase NCKAP3 Phosphatidylinositol phospholipase C Phosphoinositidase C Phosphoinositide phospholipase C Phosphoinositide phospholipase C-gamma-1 Phospholipase C 148 Phospholipase C gamma 1 Phospholipase C-gamma-1 Phospholipase C-II PLC gamma 1 PLC II PLC-148 PLC-gamma-1 PLC-II PLC1 PLC148 Plcg1 PLCG1_HUMAN PLCgamma1
Images
ET1611-25_1.jpg Fig1: Western blot analysis of Phospholipase C gamma 1 on different lysates with Rabbit anti-Phospholipase C gamma 1 antibody (ET1611-25) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: PC-12 cell lysate (20 µg/Lane)
Lane 3: Mouse lung tissue lysate (40 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 5: Rat lung tissue lysate (40 µg/Lane)
Lane 6: Rat brain tissue lysate (40 µg/Lane)
Lane 7: NIH/3T3 cell lysate (20 µg/Lane)

Predicted band size: 149 kDa
Observed band size: 149 kDa

Exposure time: 50 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-25) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1611-25_2.jpg Fig2: Immunocytochemistry analysis of PC-12 cells labeling Phospholipase C gamma 1 with Rabbit anti-Phospholipase C gamma 1 antibody (ET1611-25) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospholipase C gamma 1 antibody (ET1611-25) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1611-25_3.jpg Fig3: ICC staining of Phospholipase C gamma 1 in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-25, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-25_4.jpg Fig4: ICC staining of Phospholipase C gamma 1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-25, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-25_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Phospholipase C gamma 1 antibody (ET1611-25) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-25) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-25_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Phospholipase C gamma 1 antibody (ET1611-25) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-25) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.