Phospho-Synapsin I (S9) Recombinant Rabbit Monoclonal Antibody [SN63-04]
cat.: ET1611-26
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SN63-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 74 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser9 of Human Synapsin I aa 1-50 / 705. |
| Positive control: | Mouse brain tissue lysates, rat brain tissue, mouse brain tissue. |
| Subcellular location: | Cell junction, Golgi apparatus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P17600 Human | O88935 Mouse | P09951 Rat |
| Alternative names: | Brain protein 4.1 SYN 1 SYN 1a SYN 1b SYN I SYN1 SYN1_HUMAN SYN1a SYN1b Synapsin 1 Synapsin I Synapsin-1 Synapsin1 SynapsinI SYNI Phospho-Synapsin Ⅰ (S9) Phospho Synapsin Ⅰ (S9) Phospho-Synapsin Ⅰ (S9) |
Images
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Fig1:
Western blot analysis of Phospho-Synapsin I (S9) on mouse brain tissue lysates with Rabbit anti-Phospho-Synapsin I (S9) antibody (ET1611-26) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 74 kDa Observed band size: 77 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-26) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-Synapsin I (S9) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-Synapsin I (S9) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.