Integrin alpha E Recombinant Rabbit Monoclonal Antibody [SN62-06]
cat.: ET1611-27
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SN62-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 130 kD |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Integrin alpha E aa 1-50 / 1,179. |
| Positive control: | Human tonsil tissue, human lymph node tissue, human small intestine tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
IHC-P |
1:50-1:1000 |
| Uniprot #: | SwissProt: P38570 Human |
| Alternative names: | A530055J10 alpha-E1 alpha-M290 aM290 Antigen CD103 CD 103 CD103 CD103 antigen HML 1 antigen HML-1 antigen HML1 antigen Human mucosal lymphocyte antigen 1 Human mucosal lymphocyte antigen 1, alpha subunit Huminae Integrin alpha E heavy chain Integrin alpha E light chain Integrin alpha E, epithelial-associated Integrin alpha E1 Integrin alpha IEL Integrin alpha M290 Integrin alpha-E heavy chain Integrin alpha-IEL Integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) Integrin, alpha E ITAE_HUMAN Itgae MGC141996 MGC183601 Mucosal lymphocyte 1 antigen Mucosal lymphocyte antigen 1 |
Images
|
Fig1: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha E antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human lymph node tissue using anti-Integrin alpha E antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-27, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Integrin alpha E antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-27, 1/1000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.