Angiopoietin 1 Recombinant Rabbit Monoclonal Antibody [SN67-02]
cat.: ET1611-28
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | SN67-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Angiopoietin 1 aa 249-300 / 498. |
| Positive control: | U-87 MG cell lysate, HeLa cell lysate, K-562 cell lysate, C6 cell lysate, RAW264.7 cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q15389 Human | O08538 Mouse | O35460 Rat |
| Alternative names: | AGP 1 AGP1 AGPT ANG 1 ANG ANG-1 ANG1 Angiopoietin-1 Angiopoietin1 ANGP1_HUMAN ANGPT 1 Angpt1 KIAA0003 OTTHUMP00000227592 OTTHUMP00000227599 OTTHUMP00000227600 |
Images
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Fig1:
Western blot analysis of Angiopoietin 1 on different lysates with Rabbit anti-Angiopoietin 1 antibody (ET1611-28) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: C6 cell lysate Lane 5: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 65 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Angiopoietin 1 on different lysates with Rabbit anti-Angiopoietin 1 antibody (ET1611-28) at 1/2,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD Angiopoietin 1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 58 kDa Observed band size: 65 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-28) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.