Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | SN67-02 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 58 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Angiopoietin 1 aa 249-300 / 498. |
Positive control: | K562 cell lysates. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB |
1:500-1:2,000 |
Uniprot #: | SwissProt: Q15389 Human | O35460 Rat |
Alternative names: | AGP 1 AGP1 AGPT ANG 1 ANG ANG-1 ANG1 Angiopoietin-1 Angiopoietin1 ANGP1_HUMAN ANGPT 1 Angpt1 KIAA0003 OTTHUMP00000227592 OTTHUMP00000227599 OTTHUMP00000227600 |
Fig1: Western blot analysis of Angiopoietin 1 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |