Phospho-PDGFR beta (Y740) Recombinant Rabbit Monoclonal Antibody [SN0646]
cat.: ET1611-29
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SN0646 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 124 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Tyr740 of human PDGFR beta. |
| Positive control: | NIH/3T3 treated with 50 ng/mL PDGF for 5 minutes whole cell lysate, C2C12, Hela, L6. |
| Subcellular location: | Cell membrane, Cytoplasmic vesicle. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue |
1:1,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P09619 Human | P05622 Mouse | Q05030 Rat |
| Alternative names: | Beta platelet derived growth factor receptor Beta-type platelet-derived growth factor receptor CD 140B CD140 antigen-like family member B CD140b CD140b antigen IBGC4 IMF1 JTK12 OTTHUMP00000160528 PDGF R beta PDGF-R-beta PDGFR 1 PDGFR PDGFR beta PDGFR1 PDGFRB PGFRB_HUMAN Platelet derived growth factor receptor 1 Platelet derived growth factor receptor beta Platelet derived growth factor receptor beta polypeptide |
Images
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Fig1:
Western blot analysis of Phospho-PDGFR beta (Y740) on different lysates with Rabbit anti-Phospho-PDGFR beta (Y740) antibody (ET1611-29) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 50 ng/mL PDGF for 5 minutes whole cell lysate Lane 3: NIH/3T3 treated with λpp whole cell lysate Lane 4: NIH/3T3 treated with 50 ng/mL PDGF for 5 minutes then treated with λpp whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 124 kDa Observed band size: 180 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-29) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining PDGF Receptor beta(phospho Y740) in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining PDGF Receptor beta(phospho Y740) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining PDGF Receptor beta(phospho Y740) in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.