RDC1 Recombinant Rabbit Monoclonal Antibody [SN65-09]
cat.: ET1611-3
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | SN65-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human RDC1 aa 313-362 / 362. |
| Positive control: | PC-3M cell lysates, HUVEC cell lysate, Jurkat cell lysate, Jurkat. |
| Subcellular location: | Cell membrane, Early endosome, Recycling endosome, perinuclear region. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:10-1:100 |
| Uniprot #: | SwissProt: P25106 Human | P56485 Mouse | O89039 Rat |
| Alternative names: | ACKR3 atypical chemokine receptor 3 C X C chemokine receptor type 7 C-X-C chemokine receptor type 7 Chemokine (C-X-C motif) receptor 7 Chemokine C X C motif receptor 7 Chemokine orphan receptor 1 CMKOR1 CXC R7 CXC-R7 CXCR 7 CXCR-7 CXCR7 CXCR7_HUMAN G protein coupled receptor 159 G protein coupled receptor G protein coupled receptor RDC1 homolog G-protein coupled receptor 159 G-protein coupled receptor RDC1 homolog GPR159 GPRN1 RDC 1 RDC-1 RDC1 |
Images
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Fig1:
Western blot analysis of RDC1 on PC-3M cell lysates with Rabbit anti-RDC1 antibody (ET1611-3) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-3) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of RDC1 on different lysates with Rabbit anti-RDC1 antibody (ET1611-3) at 1/500 dilution. Lane 1: HUVEC cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-3) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3: Flow cytometric analysis of RDC1 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-3, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.