Phospho-SIRT1 (S47) Recombinant Rabbit Monoclonal Antibody [SN06-40]
cat.: ET1611-31
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SN06-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 82 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser47 of Human SIRT1 aa 30-79 / 747. |
| Positive control: | 293T whole cell lysate, 293T treated with 100nM Calyculin A for15 minutes whole cell lysate, A549, HepG2, SW480, human testis tissue, human colon tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:2,000 1:100-1:500 1:50-1:200 |
| Uniprot #: | SwissProt: Q96EB6 Human |
| Alternative names: | 75SirT1 hSIR2 hSIRT1 HST2, S. cerevisiae, homolog of NAD dependent deacetylase sirtuin 1 NAD dependent protein deacetylase sirtuin 1 OTTHUMP00000198111 OTTHUMP00000198112 Regulatory protein SIR2 homolog 1 SIR1_HUMAN SIR2 SIR2 like 1 SIR2 like protein 1 SIR2, S.cerevisiae, homolog-like 1 SIR2-like protein 1 SIR2ALPHA SIR2L1 Sirt1 SirtT1 75 kDa fragment Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) Sirtuin 1 Sirtuin type 1 |
Images
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Fig1:
Western blot analysis of Phospho-SIRT1 (S47) on different lysates with Rabbit anti-Phospho-SIRT1 (S47) antibody (ET1611-31) at 1/2,000 dilution. Lane 1: 293T whole cell lysate Lane 2: 293T treated with 100nM Calyculin A for15 minutes whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 82 kDa Observed band size: 130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-31) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining Phospho-SIRT1 (S47) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Phospho-SIRT1 (S47) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Phospho-SIRT1 (S47) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Phospho-SIRT1 (S47) antibody (ET1611-31) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-31) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Phospho-SIRT1 (S47) antibody (ET1611-31) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-31) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.