SHP2 Recombinant Rabbit Monoclonal Antibody [SN72-02]
cat.: ET1611-35
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SN72-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human SHP2. |
| Positive control: | K-562 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, mouse heart tissue lysate, rat brain tissue lysate, rat heart tissue lysate, human tonsil tissue, mouse testis tissue, rat testis tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IP IHC-P |
1:1,000-1:5,000 Use at an assay dependent concentration. 1:500 |
| Uniprot #: | SwissProt: Q06124 Human | P35235 Mouse | P41499 Rat |
| Alternative names: | BPTP3 CFC JMML METCDS MGC14433 NS1 OTTHUMP00000166107 OTTHUMP00000166108 Protein tyrosine phosphatase 2 Protein tyrosine phosphatase 2C Protein tyrosine phosphatase non receptor type 11 Protein-tyrosine phosphatase 1D Protein-tyrosine phosphatase 2C PTN11_HUMAN PTP-1D PTP-2C PTP1D PTP2C PTPN11 SAP2 SH-PTP2 SH-PTP3 SH2 domain containing protein tyrosine phosphatase 2 SHP 2 SHP-2 Shp2 SHPTP2 SHPTP3 Syp Tyrosine-protein phosphatase non-receptor type 11 |
Images
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Fig1:
Western blot analysis of SHP2 on different lysates with Rabbit anti-SHP2 antibody (ET1611-35) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: Mouse heart tissue lysate (40 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Lane 7: Rat heart tissue lysate (40 µg/Lane) Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-35) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of SHP2 on different lysates with Rabbit anti-SHP2 antibody (ET1611-35) at 1/2,000 dilution. Lane 1: SU-DHL-6-si NT cell lysate Lane 2: SU-DHL-6-si SHP2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-35) at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-SHP2 antibody (ET1611-35) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-35) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SHP2 antibody (ET1611-35) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-35) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-SHP2 antibody (ET1611-35) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-35) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.