AGR2 Recombinant Rabbit Monoclonal Antibody [SN74-01]
cat.: ET1611-36
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: SN74-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 20 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human AGR2 aa 1-50 / 175.
Positive control: NIH/3T3 cell lysate, MCF-7 cell lysate, Hela cell lysate, Hela, MCF-7, NIH/3T3, human colon tissue.
Subcellular location: Secreted, Endoplasmic reticulum.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
  IP
1:1,000-1:5,000
1:100-1:500
1:1,000
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: O95994 Human | O88312 Mouse
Alternative names: AG 2 AG 2 protein AG-2 AG2 AG2 protein AGR 2 AGR2 AGR2_HUMAN Anterior gradient 2 homolog (Xenopus laevis) anterior gradient 2 homolog Anterior gradient 2, Xenopus, homolog of Anterior gradient homolog 2 Anterior gradient protein 2 homolog GOB 4 GOB4 HAG 2 hAG-2 hAG2 HAG2/R HPC 8 hPC8 PDIA17 Protein disulfide isomerase family A member 17 secreted cement gland homolog Secreted cement gland protein XAG 2 homolog Secreted cement gland protein XAG-2 homolog Secreted cement gland protein XAG2 homolog XAG 2 XAG2
Images
ET1611-36_1.jpg Fig1: Western blot analysis of AGR2 on different lysates with Rabbit anti-AGR2 antibody (ET1611-36) at 1/1,000 dilution.

Lane 1: A549-si NT cell lysate
Lane 2: A549-si AGR2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 20 kDa
Observed band size: 15 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-36) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1611-36_2.jpg Fig2: Western blot analysis of AGR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-36, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: NIH/3T3 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: Hela cell lysate
ET1611-36_3.jpg Fig3: ICC staining of AGR2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-36, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-36_4.jpg Fig4: ICC staining of AGR2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-36, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-36_5.jpg Fig5: ICC staining of AGR2 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-36, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-36_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-AGR2 antibody (ET1611-36) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-36) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-36_7.jpg Fig7: Flow cytometric analysis of AGR2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-36, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.