NSD3 Recombinant Rabbit Monoclonal Antibody [SN07-11]
cat.: ET1611-39
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SN07-11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 162 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NSD3 aa 1-50 / 1,437. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat eyeball tissue lysate, human small intestine tissue, human lymph nodes tissue, Hela. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB FC IHC-P |
1:10,000 1:50-1:100 1:50-1:400 |
| Uniprot #: | SwissProt: Q9BZ95 Human | Q6P2L6 Mouse | D3ZK47 |
| Alternative names: | DKFZp667H044 FLJ20353 Histone lysine N methyltransferase NSD3 Histone-lysine N-methyltransferase NSD3 MGC126766 MGC142029 NSD 3 NSD3_HUMAN Nuclear set domain containing 3 Nuclear SET domain containing protein 3 Nuclear SET domain-containing protein 3 pp14328 Protein whistle Whistle WHSC1 like protein 1 WHSC1-like 1 isoform 9 with methyltransferase activity to lysine WHSC1-like protein 1 WHSC1L1 WHSC1L1 protein Wolf Hirschhorn syndrome candidate 1 like 1 Wolf-Hirschhorn syndrome candidate 1-like protein 1 |
Images
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Fig1:
Western blot analysis of NSD3 on different lysates with Rabbit anti-NSD3 antibody (ET1611-39) at 1/10,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Rat brain tissue lysate Lane 5: Rat eyeball tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 162 kDa Observed band size: 162 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-39) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-NSD3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-39, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-NSD3 antibody (ET1611-39) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-39) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of NSD3 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-39, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.