BTK Recombinant Rabbit Monoclonal Antibody [SN69-07]
cat.: ET1611-4
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SN69-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 76 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human BTK aa 610-659 / 659. |
| Positive control: | Daudi cell lysate, Raji cell lysate, U937 cell lysate, U2932, human tonsil tissue. |
| Subcellular location: | Cell membrane, Cytoplasm, Membrane, Nucleus. |
| Recommended Dilutions:
WB IHC-P IP |
1:500-1:2,000 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q06187 Human |
| Alternative names: | Agammaglobulinaemia tyrosine kinase AGMX 1 AGMX1 AT ATK B cell progenitor kinase B-cell progenitor kinase BPK Bruton agammaglobulinemia tyrosine kinase Bruton tyrosine kinase Bruton’s Tyrosine Kinase Btk BTK_HUMAN dominant-negative kinase-deficient Brutons tyrosine kinase IMD 1 IMD1 MGC126261 MGC126262 OTTHUMP00000063593 PSCTK 1 PSCTK1 truncated Bruton agammaglobulinemia tyrosine kinase Tyrosine protein kinase BTK Tyrosine-protein kinase BTK tyrosine-protein kinase BTK isoform (lacking exon 14 XLA |
Images
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Fig1:
All lanes: Western blot analysis of BTK with anti-BTK antibody [SN69-07] (ET1611-4) at 1/1,000 dilution. Lane 1: Wild-type U2932 whole cell lysate. Lane 2: BTK knockout U2932 whole cell lysate. ET1611-4 was shown to specifically react with BTK in wild-type U2932 cells. No band was observed when BTK knockout sample was tested. Wild-type and BTK knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-BTK antibody (ET1611-4, 1/1,000) and Anti-Vinculin antibody (ET1705-94, 1/5,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China). |
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Fig2:
Western blot analysis of BTK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Daudi cell lysate Lane 2: Raji cell lysate Lane 3: U937 cell lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BTK antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-4, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.