BTK Recombinant Rabbit Monoclonal Antibody [SN69-07]
cat.: ET1611-4
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SN69-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 76 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human BTK aa 610-659 / 659. |
| Positive control: | Ramos cell lysate, Raji cell lysate, THP-1 cell lysate, Daudi cell lysate, RAW264.7 cell lysate, human tonsil tissue. |
| Subcellular location: | Cell membrane, Cytoplasm, Membrane, Nucleus. |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q06187 Human | P35991 Mouse |
| Alternative names: | Agammaglobulinaemia tyrosine kinase AGMX 1 AGMX1 AT ATK B cell progenitor kinase B-cell progenitor kinase BPK Bruton agammaglobulinemia tyrosine kinase Bruton tyrosine kinase Bruton’s Tyrosine Kinase Btk BTK_HUMAN dominant-negative kinase-deficient Brutons tyrosine kinase IMD 1 IMD1 MGC126261 MGC126262 OTTHUMP00000063593 PSCTK 1 PSCTK1 truncated Bruton agammaglobulinemia tyrosine kinase Tyrosine protein kinase BTK Tyrosine-protein kinase BTK tyrosine-protein kinase BTK isoform (lacking exon 14 XLA |
Images
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Fig1:
Western blot analysis of BTK on different lysates with Rabbit anti-BTK antibody (ET1611-4) at 1/5,000 dilution. Lane 1: Ramos cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: Raji cell lysate Lane 4: THP-1 cell lysate Lane 5: Daudi cell lysate Lane 6: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 76 kDa Observed band size: 72 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-4) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
All lanes: Western blot analysis of BTK with anti-BTK antibody [SN69-07] (ET1611-4) at 1/1,000 dilution. Lane 1: Wild-type U2932 whole cell lysate. Lane 2: BTK knockout U2932 whole cell lysate. ET1611-4 was shown to specifically react with BTK in wild-type U2932 cells. No band was observed when BTK knockout sample was tested. Wild-type and BTK knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-BTK antibody (ET1611-4, 1/1,000) and Anti-Vinculin antibody (ET1705-94, 1/5,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BTK antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-4, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.