NEDD4L Recombinant Rabbit Monoclonal Antibody [SN73-03]
cat.: ET1611-42
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | SN73-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 112 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NEDD4L aa 926-975 / 975. |
| Positive control: | Jurkat cell lysate, HT-29 cell lysate, HCT 116 cell lysate, SW480 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, L6 cell lysate. |
| Subcellular location: | Cytoplasm, Endosome, Golgi apparatus. |
| Recommended Dilutions:
WB IP |
1:1,000-1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q96PU5 Human | Q8CFI0 Mouse Unigene: 72824 Rat |
| Alternative names: | E3 ubiquitin protein ligase NEDD4 like protein E3 ubiquitin-protein ligase NEDD4-like KIAA0439 NED4L_HUMAN NEDD4 2 NEDD4-2 NEDD4 2c Nedd4-2 NEDD4-2a NEDD4.2 NEDD4b NEDD4L NEDD4La NEDD4Lb NEDD4Lc NEDD4Ld NEDD4Le NEDD4Lf NEDD4Lg NEDD4Lh NEDL3 Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase Neural precursor cell expressed, developmentally down regulated 4 like RSP5 Ubiquitin protein ligase Rsp5 |
Images
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Fig1:
Western blot analysis of NEDD4L on different lysates with Rabbit anti-NEDD4L antibody (ET1611-42) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (20 µg/Lane) Lane 3: HCT 116 cell lysate (20 µg/Lane) Lane 4: SW480 cell lysate (20 µg/Lane) Predicted band size: 112 kDa Observed band size: 130 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of NEDD4L on different lysates with Rabbit anti-NEDD4L antibody (ET1611-42) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate (15 µg/Lane) Lane 2: C2C12 cell lysate (15 µg/Lane) Lane 3: C6 cell lysate (15 µg/Lane) Lane 4: L6 cell lysate (15 µg/Lane) Predicted band size: 112 kDa Observed band size: 130 kDa Exposure time: 17 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
NEDD4L was immunoprecipitated from 0.2 mg Jurkat cell lysate with ET1611-42 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1611-42 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: ET1611-42 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of ET1611-42 in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.