NOX2/gp91phox Recombinant Rabbit Monoclonal Antibody [SN07-16]
cat.: ET1611-44
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | SN07-16 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 65 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NOX2 aa 131-180 / 570. |
| Positive control: | HepG2 cell lysate, MCF7 cell lysate, mouse brain tissue lysate, mouse spleen tissue lysate, rat brain tissue lysate, rat spleen tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P04839 Human | Q61093 Mouse Entrez Gene: 66021 Rat |
| Alternative names: | AMCBX2 CGD CGD91-phox CY24B_HUMAN CYBB Cytochrome b 245, beta polypeptide Cytochrome b(558) beta chain Cytochrome b(558) subunit beta Cytochrome b-245 heavy chain Cytochrome b558 subunit beta GP91 PHOX gp91-1 gp91-phox GP91PHOX Heme-binding membrane glycoprotein gp91phox NADPH oxidase 2 Neutrophil cytochrome b 91 kDa polypeptide NOX2 p22 phagocyte B-cytochrome P91 PHOX p91-PHOX Superoxide-generating NADPH oxidase heavy chain subunit |
Images
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Fig1:
Western blot analysis of NOX2/gp91phox on different lysates with Rabbit anti-NOX2/gp91phox antibody (ET1611-44) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane) Lane 2: MCF7 cell lysate (15 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Mouse spleen tissue lysate (20 µg/Lane) Lane 5: Rat brain tissue lysate (20 µg/Lane) Lane 6: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 65 kDa Observed band size: 55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-44) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.