Histone H2B (acetyl K20) Recombinant Rabbit Monoclonal Antibody [SN72-09]
cat.: ET1611-48
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC, IP, ChIP, CUT&Tag-seq |
| Clonality: | Monoclonal |
| Clone number: | SN72-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Histone H2B aa 1-49/126. |
| Positive control: | SW480, MCF-7, Hela, human tonsil tissue, human kidney tissue, human colon tissue, mouse colon tissue, human colon cancer tissue, mouse kidney tissue, human breast carcinoma tissue. |
| Subcellular location: | Chromosome, Nucleosome core, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP ChIP |
1:1,000 1:100-1:500 1:100-1:500 1:100-1:500 1:50-1:100 Use at an assay dependent concentration. Use 0.5~2 μg for 25 μg of chromatin. |
| Uniprot #: | SwissProt: O60814 Human | P06899 Human | P23527 Human | P33778 Human | P57053 Human | P58876 Human | P62807 Human | Q16778 Human | Q5QNW6 Human | Q8N257 Human | Q93079 Human | Q99877 Human | Q99879 Human | Q99880 Human Entrez Gene: 64647 Mouse | Q00729 Rat |
| Alternative names: | H2B 1A H2B H2B histone family H2B2f H2B2F_HUMAN H2Ba H2Bf HIST2H2BF histone H2B histone H2B type 1 Histone H2B type 2-F MGC131639 H2BK20ac |
Images
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Fig1: ICC staining Histone H2B(acetyl K20) (1/500) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining Histone H2B(acetyl K20) (1/50) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Histone H2B(acetyl K20) (1/200) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin. |
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Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin. |
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Fig10: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin. |
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Fig11: Flow cytometric analysis of Hela cells with Histone H2B(acetyl K20) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was use |
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Fig12: Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with 500ng/mL TSA for 4 hours and either Histone H2B (acetyl K20) (ET1611-48) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.