HA tag Recombinant Rabbit Monoclonal Antibody [SN07-06]
cat.: ET1611-49
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | SN07-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide. |
| Positive control: | HA tag recombinant protein, C-terminal HA-tagged recombinant protein. |
| Recommended Dilutions:
WB IP |
1:1,000-1:5,000 2-5 µg/ml. |
| Alternative names: | HA epitope tag HA1 HA2 hemagglutinin Hemagglutinin HA1 chain Hemagglutinin HA2 chain |
Images
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Fig1: Western blot analysis of HA tag on HA tag recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-49, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of HA tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-49, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: C-terminal HA-tagged recombinant protein Lane 2: N-terminal HA-tagged recombinant protein |
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Fig3:
HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with ET1611-49 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1008-1 at 1/1,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature. Lane 1: HA Tag fusion protein lysate (input). Lane 2: ET1611-49 IP in HA Tag fusion protein lysate. Lane 3: 0906-1 IP in HA Tag fusion protein lysate. Lane 4: Rabbit IgG instead of ET1611-49 in HA Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.