HA tag Recombinant Rabbit Monoclonal Antibody [SN07-06]
cat.: ET1611-49
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IP, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SN07-06 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N Terminal fusion protein and C Terminal fusion protein. |
| Positive control: | N-terminal HA-tagged recombinant protein, C-terminal HA-tagged recombinant protein. |
| Recommended Dilutions:
WB IP IF-Cell |
1:5,000 2-5 µg/ml. 1:1,000 |
| Alternative names: | HA epitope tag HA1 HA2 hemagglutinin Hemagglutinin HA1 chain Hemagglutinin HA2 chain |
Images
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Fig1:
Western blot analysis of HA tag on different lysates with Rabbit anti-HA tag antibody (ET1611-49) at 1/5,000 dilution. Lane 1: N-terminal HA-tagged recombinant protein Lane 2: C-terminal HA-tagged recombinant protein Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1611-49, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 30 kDa Observed band size: 30 kDa |
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Fig2:
Application: Immunocytochemistry (IF-cell) Species: Human Sample: 293T cells transfected with HA-tagged empty control/ 293T cells transfected with HA-tagged CLK4 (C-terminal) expression vector/ 293T cells transfected with HA-tagged Nanos homolog 3 (N-terminal) expression vector Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: ET1611-49, 1/1,000, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
|
Fig3:
HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with ET1611-49 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1008-1 at 1/5,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/20,000 dilution was used for 60 mins at room temperature. Lane 1: HA Tag fusion protein lysate (input). Lane 2: ET1611-49 IP in HA Tag fusion protein lysate. Lane 3: 0906-1 IP in HA Tag fusion protein lysate. Lane 4: Rabbit IgG instead of ET1611-49 in HA Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.