ET1611-50

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, IF-Cell, IF-Tissue
Clonality:	Monoclonal
Clone number:	SN07-36

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Protein A affinity purified.

Molecular weight:

Predicted band size: 134 kDa

Isotype:

IgG

Immunogen:

Synthetic phospho-peptide corresponding to residues surrounding Ser695 of human EGFR.

Positive control:

HUVEC, A549, A431.

Subcellular location:

Cell membrane, Nucleus, Endoplasmic reticulum membrane, Golgi apparatus membrane, Endosome, Secreted.

Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue

1:1,000
1:100-1:500
1:100-1:500

Uniprot #:

SwissProt: P00533 Human

Alternative names:

Avian erythroblastic leukemia viral (v erb b) oncogene homolog Cell growth inhibiting protein 40 Cell proliferation inducing protein 61 EGF R EGFR EGFR_HUMAN Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) Epidermal growth factor receptor erb-b2 receptor tyrosine kinase 1 ERBB ERBB1 Errp HER1 mENA NISBD2 Oncogen ERBB PIG61 Proto-oncogene c-ErbB-1 Receptor tyrosine protein kinase ErbB 1 Receptor tyrosine-protein kinase ErbB-1 SA7 Species antigen 7 Urogastrone v-erb-b Avian erythroblastic leukemia viral oncogen homolog wa2 Wa5

	Fig1: ICC staining Phospho-EGFR(S695) (1/50) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig2: ICC staining Phospho-EGFR(S695) (1/50) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining Phospho-EGFR(S695) (1/50) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.