Phospho-Cdk2 (Y15) Recombinant Rabbit Monoclonal Antibody [SN72-04]
cat.: ET1611-52
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP, IF-Tissue
Clonality: Monoclonal
Clone number: SN72-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 34 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Tyr15 of human Cdk2.
Positive control: Hela cell lysate, NIH/3T3 cell lysate, mouse spleen tissue lysate, human breast carcinoma tissue, mouse small intestine tissue.
Subcellular location: Cytoplasm, Cytoskeleton, Endosome, Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IP
  IF-Tissue

1:1,000
1:200-1:1,000
Use at an assay dependent concentration.
1:50-1:200
Uniprot #: SwissProt: P24941 Human | P97377 Mouse | Q63699 Rat
Alternative names: Cdc2 related protein kinase cdc2-related protein kinase CDC28 CDC2A Cdk 2 CDK1 CDK2 CDK2_HUMAN CDKN2 Cell devision kinase 2 Cell division protein kinase 2 Cyclin dependent kinase 2 cyclin dependent kinase 2-alpha Cyclin-dependent kinase 2 kinase Cdc2 MPF p33 protein kinase p33(CDK2)
Images
ET1611-52_1.jpg Fig1: Western blot analysis of Phospho-Cdk2 (Y15) on different lysates with Rabbit anti-Phospho-Cdk2 (Y15) antibody (ET1611-52) at 1/1,000 dilution.

Lane 1: Hela cell lysate (10 µg/Lane)
Lane 2: NIH/3T3 cell lysate (10 µg/Lane)
Lane 3: Mouse spleen tissue lysate (20 µg/Lane)

Predicted band size: 34 kDa
Observed band size: 35 kDa

Exposure time: 1 minute;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-52) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1611-52_2.jpg Fig2: Western blot analysis of Phospho-Cdk2(Y15) on Hela cell lysates.

Lane 1: Hela cells, whole cell lysate, 10ug/lane
Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane

All lanes :
Anti-Phospho-Cdk2(Y15) antibody (ET1611-52) at 1/500 dilution. Anti-Cdk2 antibody (ET1602-6) at 1/500 dilution. Anti-GAPDH antibody (ET1601-4) at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution.

Predicted band size: 34 kDa
Observed band size: 34 kDa

Blocking and diluting buffer: 5% BSA.

Exposure time: 30 seconds
ET1611-52_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Phospho-Cdk2 (Y15) antibody (ET1611-52) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-52) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-52_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Phospho-Cdk2 (Y15) antibody (ET1611-52) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-52) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-52_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Phospho-Cdk2 (Y15) antibody (ET1611-52) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-52) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.