Phospho-Cdk2 (Y15) Recombinant Rabbit Monoclonal Antibody [SN72-04]
cat.: ET1611-52
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SN72-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Tyr15 of human Cdk2. |
| Positive control: | Hela cell lysate, NIH/3T3 cell lysate, mouse spleen tissue lysate, human breast carcinoma tissue, mouse small intestine tissue. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Endosome, Nucleus. |
| Recommended Dilutions:
WB IHC-P IP IF-Tissue |
1:1,000 1:200-1:1,000 Use at an assay dependent concentration. 1:50-1:200 |
| Uniprot #: | SwissProt: P24941 Human | P97377 Mouse | Q63699 Rat |
| Alternative names: | Cdc2 related protein kinase cdc2-related protein kinase CDC28 CDC2A Cdk 2 CDK1 CDK2 CDK2_HUMAN CDKN2 Cell devision kinase 2 Cell division protein kinase 2 Cyclin dependent kinase 2 cyclin dependent kinase 2-alpha Cyclin-dependent kinase 2 kinase Cdc2 MPF p33 protein kinase p33(CDK2) |
Images
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Fig1:
Western blot analysis of Phospho-Cdk2 (Y15) on different lysates with Rabbit anti-Phospho-Cdk2 (Y15) antibody (ET1611-52) at 1/1,000 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: NIH/3T3 cell lysate (10 µg/Lane) Lane 3: Mouse spleen tissue lysate (20 µg/Lane) Predicted band size: 34 kDa Observed band size: 35 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-52) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Phospho-Cdk2(Y15) on Hela cell lysates. Lane 1: Hela cells, whole cell lysate, 10ug/lane Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Cdk2(Y15) antibody (ET1611-52) at 1/500 dilution. Anti-Cdk2 antibody (ET1602-6) at 1/500 dilution. Anti-GAPDH antibody (ET1601-4) at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 34 kDa Observed band size: 34 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 30 seconds |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Phospho-Cdk2 (Y15) antibody (ET1611-52) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-52) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Phospho-Cdk2 (Y15) antibody (ET1611-52) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-52) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Phospho-Cdk2 (Y15) antibody (ET1611-52) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-52) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.