PP2A alpha + beta Recombinant Rabbit Monoclonal Antibody [SN70-08]
cat.: ET1611-54
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, IF-Cell, IHC-P, IP
Clonality: Monoclonal
Clone number: SN70-08
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 36 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human PP2A alpha aa 260-309 / 309.
Positive control: A431 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, hybrid fish (crucian-carp) brain tissue lysates, PANC-1, Hela, 293, L6, rat kidney tissue, human tonsil tissue, human pancreas tissue, mouse brain tissue, mouse stomach tissue, mouse heart tissue.
Subcellular location: Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  IP
1:1,000-1:2,000
1:50-1:200
1:100-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: P62714 Human | P67775 Human | P62715 Mouse | P63330 Mouse | P62716 Rat | P63331 Rat
Alternative names: PP2A A PP2A alpha PP2A B PP2A beta PP2A-alpha PP2AA_HUMAN PP2Ac PP2CB PPP2CA PPP2CB Protein phosphatase 2 catalytic subunit alpha isoform Protein phosphatase 2 catalytic subunit beta isoform Replication protein C RP C RP-C Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform Serine/threonine protein phosphatase 2A catalytic subunit beta isoform Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Images
ET1611-54_1.jpg Fig1: Western blot analysis of PP2A alpha + beta on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-54, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate
Lane 2: 293T cell lysate
Lane 3: NIH/3T3 cell lysate
ET1611-54_2.jpg Fig2: Western blot analysis of PP2A alpha + beta on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-54, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1611-54_3.jpg Fig3: ICC staining of PP2A alpha + beta in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-54, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-54_4.jpg Fig4: ICC staining of PP2A alpha + beta in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-54, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-54_5.jpg Fig5: ICC staining of PP2A alpha + beta in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-54, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-54_6.jpg Fig6: ICC staining of PP2A alpha + beta in L6 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-54, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-54_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PP2A alpha + beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-54_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PP2A alpha + beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-54_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PP2A alpha + beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-54_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PP2A alpha + beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-54_11.jpg Fig11: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PP2A alpha + beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-54_12.jpg Fig12: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-PP2A alpha + beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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