ET1611-58

ACE2 Recombinant Rabbit Monoclonal Antibody [SN0754]

cat.: ET1611-58

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Hamster
Applications:	WB, IF-Cell, IHC-P, IF-Tissue, IP
Clonality:	Monoclonal
Clone number:	SN0754

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 92 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human ACE2 aa 181-230 / 805.
Positive control:	HepG2 cell lysate, 293T cell lysate, rat brain tissue lysate, rat kidney tissue lysate, human kidney tissue lysate, human small intestine tissue lysate, mouse kidney tissue lysate, mouse testis tissue lysate, hamster testis lysates, hamster stomach tissue lysates, 293, MCF-7, HepG2, human kidney tissue, human testis tissue, mouse kidney tissue.
Subcellular location:	Cell membrane, Cell projection, Cytoplasm, Membrane, Secreted.
Recommended Dilutions: WB IF-Cell IHC-P IF-Tissue IP	1:1,000-1:5,000 1:100-1:500 1:1,000 1:200 Use at an assay dependent concentration.
Uniprot #:	SwissProt: Q9BYF1 Human \| Q8R0I0 Mouse \| Q5EGZ1 Rat \| A0A1U7QTA1 Hamster
Alternative names:	ACE 2 ACE related carboxypeptidase ACE-related carboxypeptidase ACE2 ACE2_HUMAN ACEH Angiotensin converting enzyme 2 Angiotensin converting enzyme homolog Angiotensin converting enzyme like protein Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 Angiotensin I converting enzyme 2 Angiotensin-converting enzyme homolog DKFZP434A014 EC 3.4.17 metalloprotease MPROT 15 Metalloprotease MPROT15 OTTHUMP00000022963 Processed angiotensin-converting enzyme 2

Images

Fig1: Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: 293T cell lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat kidney tissue lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 92 kDa
Observed band size: 100 kDa

Exposure time: 2 minutes; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-58) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (ET1611-58) at 1/2,000 dilution.

Lane 1: Human kidney tissue lysate
Lane 2: Human small intestine tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 92 kDa
Observed band size: 100 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-58) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

	Fig3: Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate Lane 2: Mouse testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 92 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-58) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
	Fig4: Western blot analysis of ACE2 on Hamster testis (1) and stomach (2) tissue lysates using anti-ACE2 antibody at 1/1,000 dilution.
	Fig5: ICC staining of ACE2 in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

	Fig6: ICC staining of ACE2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
	Fig7: ICC staining of ACE2 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
	Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1611-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig9: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1611-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig10: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1611-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig11: Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution.

Lane 1: Caco-2-si NT cell lysate
Lane 2: Caco-2-si ACE2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 92 kDa
Observed band size: 120 kDa

Exposure time: 1 minute 23 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-58) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.