ACE2 Recombinant Rabbit Monoclonal Antibody [SN0754]
cat.: ET1611-58
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Hamster
Applications: WB, IF-Cell, IHC-P, IF-Tissue, IP
Clonality: Monoclonal
Clone number: SN0754
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 92 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human ACE2 aa 181-230 / 805.
Positive control: Caco-2 cell lysate, HepG2 cell lysate, 293T cell lysate, Mouse testis tissue lysate, Mouse lung tissue lysate, Rat testis tissue lysate, Rat lung tissue lysate, Hamster testis tissue lysates, Hamster stomach tissue lysates, 293, MCF-7, HepG2, human testis tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Subcellular location: Cell membrane, Cell projection, Cytoplasm, Membrane, Secreted.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  IF-Tissue
  IP
1:1,000-1:5,000
1:100-1:500
1:1,000
1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q9BYF1 Human | Q8R0I0 Mouse | Q5EGZ1 Rat | A0A1U7QTA1 Hamster
Alternative names: ACE 2 ACE related carboxypeptidase ACE-related carboxypeptidase ACE2 ACE2_HUMAN ACEH Angiotensin converting enzyme 2 Angiotensin converting enzyme homolog Angiotensin converting enzyme like protein Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 Angiotensin I converting enzyme 2 Angiotensin-converting enzyme homolog DKFZP434A014 EC 3.4.17 metalloprotease MPROT 15 Metalloprotease MPROT15 OTTHUMP00000022963 Processed angiotensin-converting enzyme 2
Images
ET1611-58_1.jpg Fig1: Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (ET1611-58) at 1/2,000 dilution.

Lane 1: Caco-2 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: Mouse testis tissue lysate (40 µg/Lane)
Lane 5: Mouse lung tissue lysate (40 µg/Lane)
Lane 6: Rat testis tissue lysate (40 µg/Lane)
Lane 7: Rat lung tissue lysate (40 µg/Lane)

Predicted band size: 92 kDa
Observed band size: 130 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-58) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1611-58_2.jpg Fig2: Western blot analysis of ACE2 on Hamster testis (1) and stomach (2) tissue lysates using anti-ACE2 antibody at 1/1,000 dilution.
ET1611-58_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-58_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-58_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-58_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-58_7.jpg Fig7: Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution.

Lane 1: Caco-2-si NT cell lysate
Lane 2: Caco-2-si ACE2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 92 kDa
Observed band size: 120 kDa

Exposure time: 1 minute 23 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-58) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.