Glycogen synthase 1 / GYS1 Recombinant Rabbit Monoclonal Antibody [SN75-05]
cat.: ET1611-59
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SN75-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GYS1 aa 638-737 / 737. |
| Positive control: | HEK-293 cell lysate, A431 cell lysate, RD cell lysate, Mouse heart tissue lysate, Mouse skeletal muscle tissue lysate, Rat heart tissue lysate, Rat skeletal muscle tissue lysate, human skeletal muscle tissue, mouse cardiac muscle tissue, rat cardiac muscle tissue. |
| Subcellular location: | Cytosol, Inclusion body, Membrane. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IP |
1:2,000 1:50-1:200 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P13807 Human | Q9Z1E4 Mouse | A2RRU1 Rat |
| Alternative names: | Glycogen [starch] synthase Glycogen synthase 1 (muscle) Glycogen synthase 1 GSY GYS Gys1 GYS1_HUMAN muscle |
Images
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Fig1:
Western blot analysis of Glycogen synthase 1 / GYS1 on different lysates with Rabbit anti-Glycogen synthase 1 / GYS1 antibody (ET1611-59) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Glycogen synthase 1 / GYS1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 60 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-59) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Glycogen synthase 1 / GYS1 on different lysates with Rabbit anti-Glycogen synthase 1 / GYS1 antibody (ET1611-59) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A431 cell lysate Lane 3: RD cell lysate Lane 4: Mouse heart tissue lysate Lane 5: Mouse skeletal muscle tissue lysate Lane 6: Rat heart tissue lysate Lane 7: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: Lane 1-3: 46 seconds; Lane 4-7: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-59) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Glycogen synthase 1 / GYS1 antibody (ET1611-59) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-59) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue with Rabbit anti-Glycogen synthase 1 / GYS1 antibody (ET1611-59) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-59) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue with Rabbit anti-Glycogen synthase 1 / GYS1 antibody (ET1611-59) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-59) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Glycogen synthase 1 / GYS1 was immunoprecipitated from 0.2 mg A431 cell lysate with ET1611-59 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1611-59 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input) Lane 2: ET1611-59 IP in A431 cell lysate Lane 3: Rabbit IgG instead of ET1611-59 in A431 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801 |
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