BPI Recombinant Rabbit Monoclonal Antibody [SN06-63]
cat.: ET1611-6
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Zebrafish |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | SN06-63 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human BPI aa 131-180 / 487. |
| Positive control: | THP-1 cell lysate, Jurkat cell lysate, HL-60 cell lysate, Mouse thymus tissue lysate, THP-1, Jurkat. |
| Subcellular location: | Secreted, Cytoplasmic granule membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P17213 Human | Q67E05 Mouse |
| Alternative names: | Bactericidal permeability-increasing protein bactericidal/permeability-increasing protein BPI BPI fold containing family D, member 1 BPIFD1 CAP 57 rBPI recombinant BPI holoprotein, rBPI |
Images
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Fig1:
Western blot analysis of BPI on different lysates with Rabbit anti-BPI antibody (ET1611-6) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: HL-60 cell lysate (20 µg/Lane) Lane 4: Mouse thymus tissue lysate (40 µg/Lane) Predicted band size: 54 kDa Observed band size: 60 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-6) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of THP-1 cells labeling BPI with Rabbit anti-BPI antibody (ET1611-6) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BPI antibody (ET1611-6) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3: Flow cytometric analysis of BPI was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-6, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.