CD13 Recombinant Rabbit Monoclonal Antibody [SN71-04]
cat.: ET1611-61
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SN71-04 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 110 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD13 aa 401-450 / 967. |
| Positive control: | 293T cell lysate, PANC-1 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human liver tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IP IF-Tissue |
1:1,000-1:5,000 1:2,000 1-2μg/sample 1:500 |
| Uniprot #: | SwissProt: P15144 Human | P97449 Mouse | P15684 Rat |
| Alternative names: | Alanyl (membrane) aminopeptidase Alanyl aminopeptidase Aminopeptidase M Aminopeptidase N AMPN_HUMAN ANPEP AP M AP N AP-M AP-N APN CD 13 CD13 CD13 antigen gp150 hAPN LAP 1 LAP1 Microsomal aminopeptidase Myeloid plasma membrane glycoprotein CD13 p150 PEPN |
Images
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Fig1:
Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (ET1611-61) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-CD13 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 110 kDa Observed band size: 150 kDa Exposure time: 110 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-61) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (ET1611-61) at 1/2,000 dilution. Lane 1: 293T cell lysate (negative) (20 µg/Lane) Lane 2: PANC-1 cell lysate (5 µg/Lane) Lane 3: Mouse kidney tissue lysate (20 µg/Lane) Lane 4: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 110 kDa Observed band size: 150 kDa Exposure time: 45 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-61) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CD13 antibody (ET1611-61) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-61) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
CD13 was immunoprecipitated from 0.2 mg PANC-1 cell lysate with ET1611-61 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1611-61 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: PANC-1 cell lysate (input) Lane 2: ET1611-61 IP in PANC-1 cell lysate Lane 3: Rabbit IgG instead of ET1611-61 in PANC-1 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 20 seconds; ECL: K1801 |
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Fig5:
Application: IF-Tissue Species: Mouse Site: kidney Sample: Paraffin-embedded section Antibody concentration: 1/500 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.