CCR2 Recombinant Rabbit Monoclonal Antibody [SN707]
cat.: ET1611-65
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SN707 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human CCR2. |
| Positive control: | Mouse spleen tissue lysate, THP-1 cell lysate, human tonsil tissue, human breast carcinoma tissue, human esophagus tissue, THP-1. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P FC IP |
1:500-1:1,000 1:50-1:500 1:500-1:1,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P41597 Human | P51683 Mouse |
| Alternative names: | C C chemokine receptor type 2 C C CKR 2 C-C chemokine receptor type 2 C-C CKR-2 CC chemokine receptor type 2 CC CKR 2 CC-CKR-2 CCCKR2 CCR 2 CCR-2 CCR1L CCR2 CCR2_HUMAN CCR2A CCR2B CCR5L CD192 CD192 antigen Chemokine C C motif receptor 2 Chemokine CC Motif Receptor 2 CKR 2 CKR2 CKR2A CKR2B CMKBR2 MCP 1 R MCP-1-R MCP1 RECEPTOR MCP1R Monocyte chemoattractant protein 1 receptor Monocyte Chemotactic Protein 1 Receptor Monocyte Chemotactic Protein 1 Receptor |
Images
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Fig1:
Western blot analysis of CCR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-65, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse spleen tissue lysate Lane 2: THP-1 cell lysate |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CCR2 antibody (ET1611-65) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-65) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CCR2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-65, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-CCR2 antibody (ET1611-65) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-65) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of THP-1 cells labeling CCR2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (ET1611-65, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.