CCR2 Recombinant Rabbit Monoclonal Antibody [SN707]
cat.: ET1611-65
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SN707 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human CCR2. |
| Positive control: | Mouse spleen tissue lysate, THP-1 cell lysate, human tonsil tissue, human breast carcinoma tissue, human esophagus tissue, THP-1. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P FC IP |
1:2,000 1:500 1:500-1:1,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P41597 Human | P51683 Mouse |
| Alternative names: | C C chemokine receptor type 2 C C CKR 2 C-C chemokine receptor type 2 C-C CKR-2 CC chemokine receptor type 2 CC CKR 2 CC-CKR-2 CCCKR2 CCR 2 CCR-2 CCR1L CCR2 CCR2_HUMAN CCR2A CCR2B CCR5L CD192 CD192 antigen Chemokine C C motif receptor 2 Chemokine CC Motif Receptor 2 CKR 2 CKR2 CKR2A CKR2B CMKBR2 MCP 1 R MCP-1-R MCP1 RECEPTOR MCP1R Monocyte chemoattractant protein 1 receptor Monocyte Chemotactic Protein 1 Receptor Monocyte Chemotactic Protein 1 Receptor |
Images
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Fig1:
Western blot analysis of CCR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-65, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse spleen tissue lysate Lane 2: THP-1 cell lysate |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CCR2 antibody (ET1611-65) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-65) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-CCR2 antibody (ET1611-65) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-65) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of THP-1 cells labeling CCR2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (ET1611-65, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.