FANCD2 Recombinant Rabbit Monoclonal Antibody [SN73-08]
cat.: ET1611-67
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: SN73-08
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 164 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human FANCD2 aa 191-240 / 1,451.
Positive control: HeLa cell lysate, K-562 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, HeLa, C6, mouse testis tissue, rat stomach tissue, rat testis tissue, mouse stomach tissue.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:500-1:2,000
1:50-1:100
1:100-1:500
1:50-1:200
Uniprot #: SwissProt: Q9BXW9 Human | Q80V62 Mouse | Q6IV68 Rat
Alternative names: DKFZp762A223 FA 4 FA D2 FA4 FAC D2 FACD 2 FACD FACD2 FACD2_HUMAN FAD FAD2 FANC D2 FANCD 2 FANCD FANCD2 FANCONI ANEMIA COMPLEMENTATION GROUP D Fanconi anemia complementation group D2 Fanconi anemia group D2 protein FANCONI PANCYTOPENIA TYPE 4 FLJ23826 OTTHUMP00000158853 OTTHUMP00000207925 Protein FACD2 Type 4 Fanconi pancytopenia
Images
ET1611-67_1.jpg Fig1: Western blot analysis of FANCD2 on different lysates with Rabbit anti-FANCD2 antibody (ET1611-67) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: C6 cell lysate
Lane 5: Mouse testis tissue lysate
Lane 6: Rat testis tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 164 kDa
Observed band size: 164 kDa

Exposure time: Lane 1: 8 seconds; Lane 2-3: 30 seconds; Lane 4-6: 3 minutes; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-67) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1611-67_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling FANCD2 with Rabbit anti-FANCD2 antibody (ET1611-67) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FANCD2 antibody (ET1611-67) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1611-67_3.jpg Fig3: Immunocytochemistry analysis of C6 cells labeling FANCD2 with Rabbit anti-FANCD2 antibody (ET1611-67) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FANCD2 antibody (ET1611-67) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1611-67_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-FANCD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-67_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-FANCD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-67_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-FANCD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-67_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-FANCD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.