SIRT2 Recombinant Rabbit Monoclonal Antibody [SN70-04]
cat.: ET1611-72
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Cynomolgus monkey |
| Applications: | WB, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SN70-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SIRT2 aa 335-389 / 389. |
| Positive control: | Human brain tissue lysates, MCF7 cell lysate, HeLa cell lysate, HEK-293 cell lysate, LNCaP cell lysate, Jurkat cell lysate, human brain tissue, human heart tissue. |
| Subcellular location: | Nucleus, Cytoplasm, Midbody, Chromosome, Cell projection, Myelin membrane. |
| Recommended Dilutions:
WB IP IHC-P |
1:1,000-1:2,000 Use at an assay dependent concentration. 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q8IXJ6 Human |
| Alternative names: | FLJ35621 FLJ37491 NAD dependent deacetylase sirtuin 2 NAD-dependent deacetylase sirtuin-2 NAD-dependent protein deacetylase sirtuin-2 Regulatory protein SIR2 homolog 2 Silencing information regulator 2 like Silent information regulator 2 SIR2 SIR2 like protein 2 Sir2 related protein type 2 SIR2, S. cerevisiae, homolog-loke 2 SIR2-like protein 2 SIR2L SIR2L2 SIRT2 SIRT2_HUMAN Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) Sirtuin 2 Sirtuin type 2 |
Images
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Fig1:
Western blot analysis of SIRT2 on Human brain tissue lysates with Rabbit anti-SIRT2 antibody (ET1611-72) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 40/35 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-72) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of SIRT2 on different lysates with Rabbit anti-SIRT2 antibody (ET1611-72) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lane 3: HEK-293 cell lysate Lane 4: LNCaP cell lysate Lane 5: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 40/35 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-72) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SIRT2 antibody (ET1611-72) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-72) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-SIRT2 antibody (ET1611-72) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-72) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.