Retinoic Acid Receptor alpha Recombinant Rabbit Monoclonal Antibody [SN0725]
cat.: ET1611-77
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, FC
Clonality: Monoclonal
Clone number: SN0725
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Retinoic Acid Receptor alpha aa 1-50 / 462.
Positive control: SK-Br-3 cell lysate, MCF-7 cell lysate, MCF-7.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  FC

1:50-1:2,000
1:50-1:100
Uniprot #: SwissProt: P10276 Human | P11416 Mouse
Alternative names: NR1B1 Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein Nuclear receptor subfamily 1 group B member 1 Nucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form RAR alpha RAR RAR-alpha rara RARA_HUMAN RARalpha RARalpha1 Retinoic acid nuclear receptor alpha variant 1 Retinoic acid nuclear receptor alpha variant 2 Retinoic acid receptor alpha Retinoic acid receptor alpha polypeptide
Images
ET1611-77_1.jpg Fig1: Western blot analysis of Retinoic Acid Receptor alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SK-Br-3 cell lysate
Lane 2: MCF-7 cell lysate
ET1611-77_2.jpg Fig2: Flow cytometric analysis of Retinoic Acid Receptor alpha was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-77, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.