Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, FC |
Clonality: | Monoclonal |
Clone number: | SN0725 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 51 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Retinoic Acid Receptor alpha aa 1-50 / 462. |
Positive control: | SK-Br-3 cell lysate, MCF-7 cell lysate, MCF-7. |
Subcellular location: | Nucleus, Cytoplasm. |
Recommended Dilutions:
WB FC |
1:50-1:2,000 1:50-1:100 |
Uniprot #: | SwissProt: P10276 Human | P11416 Mouse |
Alternative names: | NR1B1 Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein Nuclear receptor subfamily 1 group B member 1 Nucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form RAR alpha RAR RAR-alpha rara RARA_HUMAN RARalpha RARalpha1 Retinoic acid nuclear receptor alpha variant 1 Retinoic acid nuclear receptor alpha variant 2 Retinoic acid receptor alpha Retinoic acid receptor alpha polypeptide |
Fig1:
Western blot analysis of Retinoic Acid Receptor alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SK-Br-3 cell lysate Lane 2: MCF-7 cell lysate |
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Fig2: Flow cytometric analysis of Retinoic Acid Receptor alpha was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-77, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |