Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P |
Clonality: | Monoclonal |
Clone number: | SN0753 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 68 kDa |
Isotype: | IgG |
Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Thr560 of Human PKC zeta aa 531-580 / 592. |
Positive control: | Mouse eyeball tissue lysates, SK-Br-3 cell lysates, human breast carcinoma tissue, mouse brain tissue. |
Subcellular location: | Cell junction, Cytoplasm, Endosome, Membrane. |
Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:50-1:200 |
Uniprot #: | SwissProt: Q05513 Human | Q02956 Mouse | P09217 Rat |
Alternative names: | 14-3-3-zetaisoform AI098070 aPKCzeta C80388 EC 2.7.11.13 KPCZ_HUMAN nPKC zeta nPKC-zeta OTTHUMP00000001368 OTTHUMP00000044160 PKC 2 PKC ZETA PKC2 Pkcz PKCZETA PKM-zeta, included PRKCZ Protein kinase C zeta Protein kinase C zeta form Protein kinase C zeta type r14-3-3 R74924 zetaPKC |
Fig1: Western blot analysis of Phospho-PKC zeta (T560) on mouse eyeball tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. | |
Fig2: Western blot analysis of Phospho-PKC zeta (T560) on SK-Br-3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-PKC zeta (T560) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-78, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-PKC zeta (T560) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-78, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |