Phospho-PKC zeta (T560) Recombinant Rabbit Monoclonal Antibody [SN0753]
cat.: ET1611-78
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SN0753 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Thr560 of Human PKC zeta aa 531-580 / 592. |
| Positive control: | SK-Br-3 cell lysate, Mouse eyeball tissue lysate, C6 cell lysate, human testis tissue, mouse testis tissue, rat testis tissue. |
| Subcellular location: | Cell junction, Cytoplasm, Endosome, Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:50 |
| Uniprot #: | SwissProt: Q05513 Human | Q02956 Mouse | P09217 Rat |
| Alternative names: | 14-3-3-zetaisoform AI098070 aPKCzeta C80388 EC 2.7.11.13 KPCZ_HUMAN nPKC zeta nPKC-zeta OTTHUMP00000001368 OTTHUMP00000044160 PKC 2 PKC ZETA PKC2 Pkcz PKCZETA PKM-zeta, included PRKCZ Protein kinase C zeta Protein kinase C zeta form Protein kinase C zeta type r14-3-3 R74924 zetaPKC |
Images
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Fig1:
Western blot analysis of Phospho-PKC zeta (T560) on different lysates with Rabbit anti-Phospho-PKC zeta (T560) antibody (ET1611-78) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate (20 µg/Lane) Lane 2: Mouse eyeball tissue lysate (30 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate, the membrane treated with λpp for 1 hour (20 µg/Lane) Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-78) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Phospho-PKC zeta (T560) antibody (ET1611-78) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-78) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Phospho-PKC zeta (T560) antibody (ET1611-78) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-78) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Phospho-PKC zeta (T560) antibody (ET1611-78) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-78) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.