Bcl10 Recombinant Rabbit Monoclonal Antibody [SN74-04]
cat.: ET1611-79
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SN74-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Bcl10 aa 198-233 / 233. |
| Positive control: | HUVEC cell lysate, A549 cell lysate, Jurkat cell lysate, MCF-7, SW480, human tonsil tissue. |
| Subcellular location: | Cytoplasm, Membrane raft. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: O95999 Human |
| Alternative names: | AI132454 B cell CLL/lymphoma 10 B cell lymphoma/leukemia10 B-cell CLL/lymphoma 10 B-cell leukemia/lymphoma 10 B-cell lymphoma/leukemia 10 Bcl 10 Bcl-10 Bcl10 BCL10_HUMAN c E10 c-E10 C81403 CARD containing apoptotic signaling protein CARD containing molecule enhancing NF kappa B CARD containing molecule enhancing NF kB CARD containing molecule enhancing NF-kB CARD containing molecule enhancing NFkB CARD containing proapoptotic protein CARD like apoptotic protein CARD-containing apoptotic signaling protein CARD-containing molecule enhancing NF-kappa-B CARD-containing proapoptotic protein CARD-like apoptotic protein CARMEN Caspase recruiting domain containing protein caspase-recruiting domain-containing protein cCARMEN cE 10 cE10 CED 3/ICH 1 prodomain homologous E10 like regulator CED-3/ICH-1 prodomain homologous E10-like regulator CED3/ICH1 prodomain homologous E10 like regulator Cellular E10 Cellular homolog of vCARMEN ...... |
Images
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Fig1:
Western blot analysis of Bcl10 on different lysates with Rabbit anti-Bcl10 antibody (ET1611-79) at 1/500 dilution. Lane 1: HUVEC cell lysate Lane 2: A549 cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 26 kDa Observed band size: 31 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-79) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Bcl10 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-79, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of Bcl10 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-79, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bcl10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-79, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.