Anti-Hemoglobin subunit alpha antibody [SN70-09]
cat.: ET1611-81
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: SN70-09
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 15 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human HBA1 aa 80-120.
Positive control: Human heart tissue lysate, human spleen tissue lysate, rat heart tissue lysate, human lung tissue, human spleen tissue, mouse embryo tissue.
Subcellular location: Cytosol, membrane, extracellular region
Recommended Dilutions:
  WB
  IHC-P
  IP

1:500-1:1,000
1:10-1:50
Use at an assay dependent concentration.
Uniprot #: SwissProt: P69905 Human | P01942 Mouse | P01946 Rat
Alternative names: Alpha 1 globin antibody Alpha globin antibody Alpha one globin antibody Alpha-globin antibody HBA_HUMAN antibody HBA1 antibody HBA2 antibody Hemoglobin alpha 1 antibody Hemoglobin alpha 1 chain antibody Hemoglobin alpha 1 globin chain antibody Hemoglobin alpha 2 antibody Hemoglobin alpha chain antibody Hemoglobin subunit alpha antibody MGC126895 antibody MGC126897 antibody
Images
ET1611-81_1.jpg Fig1: Western blot analysis of Hemoglobin subunit alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: human heart tissue lysate
Lane 2: human spleen tissue lysate
Lane 3: rat heart tissue lysate
ET1611-81_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Hemoglobin subunit alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-81, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-81_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Hemoglobin subunit alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-81, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-81_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse embryo tissue using anti-Hemoglobin subunit alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-81, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.