Hemoglobin subunit alpha Recombinant Rabbit Monoclonal Antibody [SN70-09]
cat.: ET1611-81
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SN70-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 15 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HBA1 aa 71-120 / 142. |
| Positive control: | Human heart tissue lysate, human spleen tissue lysate, rat heart tissue lysate, human lung tissue, human spleen tissue, mouse embryo tissue. |
| Subcellular location: | Cytosol, membrane, extracellular region |
| Recommended Dilutions:
WB IHC-P IP |
1:500-1:1,000 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P69905 Human | P01942 Mouse | P01946 Rat |
| Alternative names: | Alpha 1 globin Alpha globin Alpha one globin Alpha-globin HBA_HUMAN HBA1 HBA2 Hemoglobin alpha 1 Hemoglobin alpha 1 chain Hemoglobin alpha 1 globin chain Hemoglobin alpha 2 Hemoglobin alpha chain Hemoglobin subunit alpha MGC126895 MGC126897 |
Images
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Fig1:
Western blot analysis of Hemoglobin subunit alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human heart tissue lysate Lane 2: human spleen tissue lysate Lane 3: rat heart tissue lysate Predicted band size: 15 kDa Observed band size: 13 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Hemoglobin subunit alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-81, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Hemoglobin subunit alpha antibody (ET1611-81) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-81) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse embryo tissue using anti-Hemoglobin subunit alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-81, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.