CD10 Recombinant Rabbit Monoclonal Antibody [SN75-07]
cat.: ET1611-82
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IHC-P, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SN75-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 86 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD10 aa 1-50 / 750. |
| Positive control: | Ramos cell lysate, Daudi cell lysate, Mouse kidney tissue lysate, Mouse small intestine tissue lysate, Rat lung tissue lysate, 293, human kidney tissue, human prostate carcinoma tissue, human small intestine tissue, human peripheral blood granulocytes. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC IHC-P IF-Tissue |
1:2,000 1:50-1:200 1:1,000 1:50-1:5,000 1:100 |
| Uniprot #: | SwissProt: P08473 Human | Q61391 Mouse | P07861 Rat |
| Alternative names: | Atriopeptidase CALLA CD10 CD10 antigen Common acute lymphocytic leukemia antigen DKFZp686O16152 EC 3.4.24.11 Enkephalinase EPN Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) Membrane metallo endopeptidase Membrane metallo endopeptidase variant 1 Membrane metallo endopeptidase variant 2 Membrane metalloendopeptidase Membrane metalloendopeptidase neutral endopeptidase enkephalinase Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 Membrane metalloendopeptidase variant 1 Membrane metalloendopeptidase variant 2 MGC126681 MGC126707 MME NEP NEP_HUMAN Neprilysin neprilysin-390 neprilysin-411 Neutral endopeptidase 24.11 Neutral endopeptidase Neutral endopeptidase, membrane-associated SFE Skin fibroblast elastase |
Images
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Fig1:
Western blot analysis of CD10 on different lysates with Rabbit anti-CD10 antibody (ET1611-82) at 1/2,000 dilution. Lane 1: Ramos cell lysate (15 µg/Lane) Lane 2: Daudi cell lysate (15 µg/Lane) Lane 3: U-2 OS cell lysate (negative) (15 µg/Lane) Lane 4: Mouse kidney tissue lysate (20 µg/Lane) Lane 5: Mouse small intestine tissue lysate (20 µg/Lane) Lane 6: Rat lung tissue lysate (20 µg/Lane) Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 24 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-82) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of 293 cells labeling CD10 with Rabbit anti-CD10 antibody (ET1611-82) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD10 antibody (ET1611-82) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling CD10 with Rabbit anti-CD10 antibody (ET1611-82) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1611-82, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD10 antibody (ET1611-82) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-82) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-CD10 antibody (ET1611-82) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-82) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-CD10 antibody (ET1611-82) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-82) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Flow cytometric analysis of human peripheral blood granulocytes labeling CD10. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (ET1611-82, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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