Caspase-6 p18 Recombinant Rabbit Monoclonal Antibody [SN07-26]
cat.: ET1611-83
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | SN07-26 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Caspase-6 p18 aa 25-182 / 293. |
| Positive control: | HCT 116 cell lysate, U-937 cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IP |
1:1,000-1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: P55212 Human |
| Alternative names: | Caspase6 Apoptotic protease Mch-2 Apoptotic protease MCH2 CASP-6 CASP6 CASP6_HUMAN Caspase 6 Caspase 6 apoptosis related cysteine protease Caspase 6, apoptosis related cysteine peptidase Caspase-6 subunit p11 Caspase-6 subunit p20 Mch2 OTTHUMP00000162742 OTTHUMP00000162743 |
Images
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Fig1:
Western blot analysis of Caspase-6 on different lysates with Rabbit anti-Caspase-6 antibody (ET1611-83) at 1/1,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si Caspase-6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-83) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Caspase-6 on different lysates with Rabbit anti-Caspase-6 antibody (ET1611-83) at 1/2,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: U-937 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-83) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.