Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, IP |
Clonality: | Monoclonal |
Clone number: | SN07-26 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 33 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human Caspase-6 p18 aa 25-182 / 293. |
Positive control: | U937 cell lysates. |
Subcellular location: | Cytoplasm. |
Recommended Dilutions:
WB |
1:1,000-1:5,000 |
Uniprot #: | SwissProt: P55212 Human |
Alternative names: | Caspase6 Apoptotic protease Mch-2 Apoptotic protease MCH2 CASP-6 CASP6 CASP6_HUMAN Caspase 6 Caspase 6 apoptosis related cysteine protease Caspase 6, apoptosis related cysteine peptidase Caspase-6 subunit p11 Caspase-6 subunit p20 Mch2 OTTHUMP00000162742 OTTHUMP00000162743 |
Fig1:
Western blot analysis of Caspase-6 p18 on U937 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 33 kDa Observed band size: 30 kDa |