Anti-Cyclin H antibody [SN20-48]
cat.: ET1611-84
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, ICC/IF, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SN20-48 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1 mg/mL. |
| Purification: | Protein A purified. |
| Molecular weight: | 38 kDa |
| Isotype: | IgG |
| Immunogen: | recombinant protein |
| Positive control: | K562, MCF-7, Hela, PC-3M, human colon cancer tissue, mouse testis tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB ICC/IF IHC-P FC |
1:1,000-1:5,000 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | P51946(Human) Q61458(Mouse) |
| Alternative names: | 6330408H09Rik antibody AI661354 antibody AV102684 antibody AW538719 antibody CAK antibody CAK complex subunit antibody ccnh antibody CCNH_HUMAN antibody CDK activating kinase antibody CDK activating kinase complex subunit antibody Cyclin dependent kinase activating kinase antibody cyclin dependent kinase activating kinase complex subunit antibody Cyclin H antibody Cyclin-H antibody CyclinH antibody MO15 associated protein antibody MO15-associated protein antibody p34 antibody p36 antibody p37 antibody |
Images
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Fig1: Western blot analysis of Cyclin H on K562 cells lysates using anti-Cyclin H antibody at 1/1,000 dilution. |
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Fig2: ICC staining Cyclin H in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Cyclin H in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Cyclin H in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cyclin H antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cyclin H antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Hela cells with Cyclin H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.