Anti-Cyclin H antibody [SN20-48]
cat.: ET1611-84
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC/IF, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: SN20-48
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 38 kDa
Isotype: IgG
Immunogen: recombinant protein
Positive control: K562, MCF-7, Hela, PC-3M, human colon cancer tissue, mouse testis tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC

1:1,000-1:5,000
1:100-1:500
1:50-1:200
1:50-1:100
Uniprot #: P51946(Human) Q61458(Mouse)
Alternative names: 6330408H09Rik antibody AI661354 antibody AV102684 antibody AW538719 antibody CAK antibody CAK complex subunit antibody ccnh antibody CCNH_HUMAN antibody CDK activating kinase antibody CDK activating kinase complex subunit antibody Cyclin dependent kinase activating kinase antibody cyclin dependent kinase activating kinase complex subunit antibody Cyclin H antibody Cyclin-H antibody CyclinH antibody MO15 associated protein antibody MO15-associated protein antibody p34 antibody p36 antibody p37 antibody
Images
ET1611-84_1.jpg Fig1: Western blot analysis of Cyclin H on K562 cells lysates using anti-Cyclin H antibody at 1/1,000 dilution.
ET1611-84_2.jpg Fig2: ICC staining Cyclin H in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1611-84_3.jpg Fig3: ICC staining Cyclin H in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1611-84_4.jpg Fig4: ICC staining Cyclin H in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1611-84_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cyclin H antibody. Counter stained with hematoxylin.
ET1611-84_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cyclin H antibody. Counter stained with hematoxylin.
ET1611-84_7.jpg Fig7: Flow cytometric analysis of Hela cells with Cyclin H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.