Cyclin H Recombinant Rabbit Monoclonal Antibody [SN20-48]
cat.: ET1611-84
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SN20-48 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Cyclin H aa 230-323 / 323. |
| Positive control: | K562 cell lysate, Jurkat cell lysate, Hela, MCF-7, PC-3M, mouse testis tissue, human colon cancer tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:1,000-1:5,000 1:100-1:500 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P51946 Human | Q61458 Mouse |
| Alternative names: | 6330408H09Rik AI661354 AV102684 AW538719 CAK CAK complex subunit ccnh CCNH_HUMAN CDK activating kinase CDK activating kinase complex subunit Cyclin dependent kinase activating kinase cyclin dependent kinase activating kinase complex subunit Cyclin H Cyclin-H CyclinH MO15 associated protein MO15-associated protein p34 p36 p37 |
Images
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Fig1:
Western blot analysis of Cyclin H on different lysates with Rabbit anti-Cyclin H antibody (ET1611-84) at 1/1,000 dilution. Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1611-84, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 37.6 kDa Observed band size: 36 kDa |
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Fig2: ICC staining Cyclin H in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Cyclin H in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Cyclin H in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cyclin H antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cyclin H antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.