TAPA1/CD81 Recombinant Rabbit Monoclonal Antibody [SN206-01]
cat.: ET1611-87
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | SN206-01 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TAPA1 / CD81 aa 161-210 / 236. |
| Positive control: | PC-12 cell lysate, JAR cell lysate, 293, A549, SH-SY5Y, PC-12, human liver tissue, mouse testis tissue, mouse lung tissue, Jurkat. |
| Subcellular location: | Cell membrane, Basolateral cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:100-1:500 1:500-1:1000 |
| Uniprot #: | SwissProt: P60033 Human | P35762 Mouse | Q62745 Rat |
| Alternative names: | 26 kDa cell surface protein TAPA 1 26 kDa cell surface protein TAPA-1 26 kDa cell surface protein TAPA1 CD 81 CD81 CD81 antigen (target of antiproliferative 1) CD81 antigen CD81 molecule CD81_HUMAN CVID6 S5.7 TAPA 1 TAPA1 Target of the antiproliferative 1 Tetraspanin 28 Tetraspanin-28 Tetraspanin28 Tspan 28 Tspan-28 Tspan28 |
Images
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Fig1:
Western blot analysis of TAPA1/CD81 on different lysates with Rabbit anti-TAPA1/CD81 antibody (ET1611-87) at 1/2,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si TAPA1/CD81 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 26 kDa Observed band size: 25 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-87) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of TAPA1/CD81 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-87, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of TAPA1/CD81 in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-87, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4:
Western blot analysis of TAPA1/CD81 on different lysates with Rabbit anti-TAPA1/CD81 antibody (ET1611-87) at 1/1,000 dilution. Lane 1: Rat liver tissue lysate Lysates/proteins at 40 µg/Lane. Exposure time:1 min 50s; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1611-87, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 26 kDa Observed band size: 26 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.