E2F2 Recombinant Rabbit Monoclonal Antibody [SN201-04]
cat.: ET1611-88
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | SN201-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human E2F2 aa 283-415 / 437. |
| Positive control: | K562 cell lysate, 293T cell lysate, HepG2 cell lysate, K562. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC |
1:1,000-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q14209 Human | P56931 Mouse Entrez Gene: 684111 Rat |
| Alternative names: | dE2F2 E2F transcription factor 2 E2F-2 E2F2 E2F2_HUMAN Transcription factor E2F2 |
Images
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Fig1:
Western blot analysis of E2F2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate |
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Fig2: Flow cytometric analysis of E2F2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-88, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.