LSP1 Recombinant Rabbit Monoclonal Antibody [SN06-25]
cat.: ET1611-9
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SN06-25 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human LSP1 aa 249-339 / 339. |
| Positive control: | Daudi cell lysates, human tonsil tissue, mouse thymus tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P33241 Human | P19973 Mouse Unigene: 101608 Rat |
| Alternative names: | 47 kDa actin binding protein 47 kDa actin-binding protein 52 kDa phosphoprotein F actin binding and cytoskeleton associated protein Leufactin (leukocyte F-actin binding protein) Leufactin Leukocyte F actin binding protein Leukocyte specific protein 1 LSP 1 Lsp1 LSP1_HUMAN Lymphocyte specific antigen WP34 Lymphocyte specific protein 1 Lymphocyte specific protein pp52 Lymphocyte-specific antigen WP34 Lymphocyte-specific protein 1 OTTHUMP00000014138 OTTHUMP00000014139 OTTHUMP00000014140 OTTHUMP00000069612 OTTHUMP00000069614 OTTHUMP00000069615 OTTHUMP00000069616 pp52 Protein pp52 S37 protein WP 34 WP34 |
Images
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Fig1: Western blot analysis of LSP1 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-9, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-LSP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-9, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using anti-LSP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-9, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.