Insulin Receptor Beta Recombinant Rabbit Monoclonal Antibody [SN20-13]
cat.: ET1611-90
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC
Clonality: Monoclonal
Clone number: SN20-13
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 156 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Insulin receptor aa 930-970.
Positive control: MCF-7 cell lysate, 293 cell lysate, mouse skeletal muscle tissue lysates, HepG2, LO2, RH-35, PANC-1.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC
1:500-1:2,000
1:100-1:500
1:100-1:500
1:1,000
Uniprot #: SwissProt: P06213 Human | P15208 Mouse | P15127 Rat
Alternative names: CD220 HHF5 HIR A INSR Insulin receptor Insulin receptor subunit alpha IR
Images
ET1611-90_1.jpg Fig1: Western blot analysis of Insulin Receptor Beta on different lysates with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/1,000 dilution.

Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si Insulin Receptor Beta cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 156 kDa
Observed band size: 95 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-90) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1611-90_2.jpg Fig2: Western blot analysis of Insulin Receptor Beta on different lysates with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/1,000 dilution.

Lane 1: MDA-MB-231 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: T-47D cell lysate
Lane 4: Rat liver tissue lysate

Cell lysates/proteins at 20 µg/Lane.
Tissue lysates/proteins at 20 µg/Lane.

Predicted band size: 156 kDa
Observed band size: 95 kDa

Exposure time: 1 minutes 2 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-90) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1611-90_3.png Fig3: Western blot analysis of Insulin Receptor Beta on mouse skeletal muscle tissue lysates with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 156 kDa
Observed band size: 95 kDa

Exposure time: 3 minutes;

4~20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-90) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1611-90_4.jpg Fig4: ICC staining of Insulin Receptor Beta in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-90, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-90_5.jpg Fig5: ICC staining of Insulin Receptor Beta in LO2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-90, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-90_6.jpg Fig6: ICC staining of Insulin Receptor Beta in RH-35 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-90, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-90_7.jpg Fig7: ICC staining of Insulin Receptor Beta in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-90, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-90_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-90) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-90_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-90) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-90_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded human liver
tissue with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-90) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.