Anti-Delta Opioid Receptor antibody [SN20-04]
cat.: ET1611-92
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat, Mouse
Applications: WB, FC
Clonality: Monoclonal
Clone number: SN20-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 40 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human OPRD1.
Positive control: HUVEC cell lysate, rat brain tissue lysate, SH-SY5Y.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  FC

1:500-1:1,000
1:50-1:100
Uniprot #: SwissProt: P41143 Human | P32300 Mouse | P33533 Rat
Alternative names: D-OR-1 antibody Delta type opioid receptor antibody Delta type opioid receptor DOR 1 antibody Delta type opioid receptor DOR1 antibody Delta-type opioid receptor antibody DOR 1 antibody DOR-1 antibody DOR1 antibody mDOR antibody Nbor antibody Opioid receptor delta 1 antibody OPRD 1 antibody OPRD antibody OPRD_HUMAN antibody Oprd1 antibody
Images
ET1611-92_1.jpg Fig1: Western blot analysis of Delta Opioid Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HUVEC cell lysate
Lane 2: rat brain tissue lysate
ET1611-92_2.jpg Fig2: Flow cytometric analysis of Delta Opioid Receptor was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-92, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.