Delta Opioid Receptor Recombinant Rabbit Monoclonal Antibody [SN20-04]
cat.: ET1611-92
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | SN20-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human OPRD1 aa 1-50 / 372. |
| Positive control: | HUVEC cell lysate, mouse brain tissue lysate, rat brain tissue lysate, SH-SY5Y. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB FC |
1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: P41143 Human | P32300 Mouse | P33533 Rat |
| Alternative names: | D-OR-1 Delta type opioid receptor Delta type opioid receptor DOR 1 Delta type opioid receptor DOR1 Delta-type opioid receptor DOR 1 DOR-1 DOR1 mDOR Nbor Opioid receptor delta 1 OPRD 1 OPRD OPRD_HUMAN Oprd1 |
Images
|
Fig1:
Western blot analysis of Delta Opioid Receptor on different lysates with Rabbit anti-Delta Opioid Receptor antibody (ET1611-92) at 1/1,000 dilution. Lane 1: HUVEC cell lysate (10 µg/Lane) Lane 2: Mouse brain tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 40 kDa Observed band size: 45 kDa Exposure time: Lane 1-2: 20 seconds; Lane 3: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-92) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of Delta Opioid Receptor was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-92, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.