| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SN20-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TWEAKR aa 80-129 / 129. |
| Positive control: | PC-3 (Human prostate cancer cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, mouse uterus tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:10,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9NP84 Human | Q9CR75 Mouse Entrez Gene: 302965 Rat |
| Alternative names: | CD 266 CD266 CD266 antigen FGF inducible 14 FGF-inducible 14 Fibroblast growth factor inducible immediate early response protein 14 Fibroblast growth factor-inducible immediate-early response protein 14 FN 14 FN14 TNFRSF 12A TNFRSF12A TNR12_HUMAN Tumor necrosis factor receptor superfamily member 12A TWEAK R Tweak receptor Tweak-receptor TweakR |
|
Fig1:
Western blot analysis of TWEAKR on different lysates with Rabbit anti-TWEAKR antibody (ET1611-93) at 1/10,000 dilution. Lane 1: PC-3 (Human prostate cancer cell) cell lysate Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1611-93, 1/10,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 14 kDa Observed band size: 20 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-TWEAKR antibody. Counter stained with hematoxylin. |