TWEAKR Recombinant Rabbit Monoclonal Antibody [SN20-07]
cat.: ET1611-93
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: SN20-07
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 14 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human TWEAKR aa 80-129 / 129.
Positive control: PC-3 (Human prostate cancer cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, mouse uterus tissue.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:10,000
1:50-1:200
Uniprot #: SwissProt: Q9NP84 Human | Q9CR75 Mouse
Entrez Gene: 302965 Rat
Alternative names: CD 266 CD266 CD266 antigen FGF inducible 14 FGF-inducible 14 Fibroblast growth factor inducible immediate early response protein 14 Fibroblast growth factor-inducible immediate-early response protein 14 FN 14 FN14 TNFRSF 12A TNFRSF12A TNR12_HUMAN Tumor necrosis factor receptor superfamily member 12A TWEAK R Tweak receptor Tweak-receptor TweakR
Images
ET1611-93_1.jpg Fig1: Western blot analysis of TWEAKR on different lysates with Rabbit anti-TWEAKR antibody (ET1611-93) at 1/10,000 dilution.

Lane 1: PC-3 (Human prostate cancer cell) cell lysate
Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate

Lysates/proteins at 10 µg/Lane.
Exposure time: 1 minute 2 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1611-93, 1/10,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 14 kDa
Observed band size: 20 kDa
ET1611-93_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-TWEAKR antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.