Tissue Factor Recombinant Rabbit Monoclonal Antibody [SN20-16]
cat.: ET1611-95
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: SN20-16
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 33 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Tissue Factor aa 30-70.
Positive control: MDA-MB-231 cell lysate, U-937 cell lysate, SH-SY5Y cell lysate, MCF7 cell lysate, U-937, human pancreas tissue, human breast carcinoma tissue, mouse pancreas tissue, rat pancreas tissue.
Subcellular location: Membrane,screted.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:1,000
1:500
1:100-1:500
1:100-1:2,000
Uniprot #: SwissProt: P13726 Human | P20352 Mouse | P42533 Rat
Alternative names: CD142 CD142 antigen Coagulation factor III (thromboplastin tissue factor) Coagulation factor III F3 FLJ17960 TF TF_HUMAN TFA Thromboplastin Tissue factor
Images
ET1611-95_1.jpg Fig1: Western blot analysis of Tissue Factor on different lysates with Rabbit anti-Tissue Factor antibody (ET1611-95) at 1/1,000 dilution.

Lane 1: MDA-MB-231 cell lysate
Lane 2: U-937 cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: MCF7 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 33 kDa
Observed band size: 50 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-95) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ET1611-95_2.jpg Fig2: Immunocytochemistry analysis of U-937 cells labeling Tissue Factor with Rabbit anti-Tissue Factor antibody (ET1611-95) at 1/500 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Tissue Factor antibody (ET1611-95) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1611-95_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Tissue Factor antibody (ET1611-95) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-95) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-95_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Tissue Factor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-95, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-95_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Tissue Factor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-95, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-95_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Tissue Factor antibody (ET1611-95) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-95) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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