SMC1A Recombinant Rabbit Monoclonal Antibody [SN20-27]
cat.: ET1611-97
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: SN20-27
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 143 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human SMC1A aa 1,184-1,233 / 1,233.
Positive control: Hela cell lysate, 293T cell lysate, Hela, A431, SW480, human breast carcinoma tissue, human lymph nodes tissue, mouse colon tissue, rat bladder tissue, rat large intestine tissue.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:500-1:2,000
1:100-1:500
1:100-1:500
1:100-1:400
Uniprot #: SwissProt: Q14683 Human | Q8NDV3 Human | Q920F6 Mouse | Q9CU62 Mouse | Q9Z1M9 Rat
Alternative names: CDLS2 DKFZp686L19178 DXhXs423e DXS423E KIAA0178 MGC138332 OTTHUMP00000061876 RP6 29D12.1 SB1.8 Segregation of mitotic chromosomes 1 Segregation of mitotic chromosomes like 1 SMC 1 SMC protein 1B SMC-1-beta SMC-1B SMC1 SMC1A SMC1alpha SMC1alpha protein SMC1B SMC1B_HUMAN SMC1BETA SMC1beta protein SMC1L1 SMC1L2 SMCB Structural maintenance of chromosome 1 like 1 protein Structural maintenance of chromosome 1 like 2 protein Structural maintenance of chromosomes 1A Structural maintenance of chromosomes 1B Structural maintenance of chromosomes protein 1B
Images
ET1611-97_1.jpg Fig1: Western blot analysis of SMC1A on different lysates with Rabbit anti-SMC1A antibody (ET1611-97) at 1/500 dilution.

Lane 1: Hela cell lysate
Lane 2: 293T cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 143 kDa
Observed band size: 143 kDa

Exposure time: 30 seconds;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-97) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1611-97_2.jpg Fig2: ICC staining of SMC1A in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-97, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-97_3.jpg Fig3: ICC staining of SMC1A in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-97, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-97_4.jpg Fig4: ICC staining of SMC1A in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-97, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-97_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-SMC1A antibody (ET1611-97) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-97) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-97_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-SMC1A antibody (ET1611-97) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-97) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-97_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SMC1A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-97, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-97_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-SMC1A antibody (ET1611-97) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-97) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-97_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-SMC1A antibody (ET1611-97) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-97) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.