Anti-Glutamate receptor 1 antibody [SD2010]
cat.: ET1612-10
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse, Rat, Human
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: SD2010
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 102 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human Glutamate receptor 1.
Positive control: Rat brain tissue lysates, mouse hippocampus tissue lysates, rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue.
Subcellular location: Cell membrane, postsynaptic cell membrane, postsynaptic density membrane, Endoplasmic reticulum membrane, Early endosome membrane, Recycling endosome membrane, dendrite, dendritic spine.
Recommended Dilutions:
  WB
  IHC-P
  IP

1:500-1:2,000
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: P42261 Human | P23818 Mouse | P19490 Rat
Alternative names: GLUR 1 antibody GLUR A antibody AMPA 1 antibody AMPA selective glutamate receptor 1 antibody AMPA-selective glutamate receptor 1 antibody GluA1 antibody GLUH1 antibody GluR K1 antibody GluR-1 antibody GluR-A antibody GluR-K1 antibody GLUR1 antibody GLURA antibody GluRK1 antibody Glutamate receptor 1 antibody Glutamate receptor ionotropic AMPA 1 antibody Glutamate receptor ionotropic antibody Glutamate receptor, ionotropic, AMPA 1 antibody Gria1 antibody GRIA1_HUMAN antibody HBGR1 antibody MGC133252 antibody OTTHUMP00000160643 antibody OTTHUMP00000165781 antibody OTTHUMP00000224241 antibody OTTHUMP00000224242 antibody OTTHUMP00000224243 antibody
Images
ET1612-10_1.jpg Fig1: Western blot analysis of Glutamate receptor 1 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1612-10_2.jpg Fig2: Western blot analysis of Glutamate receptor 1 on mouse hippocampus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1612-10_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Glutamate receptor 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-10, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-10_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-Glutamate receptor 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-10, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-10_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Glutamate receptor 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-10, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-10_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Glutamate receptor 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-10, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.