ARF1 Recombinant Rabbit Monoclonal Antibody [SD2002]
cat.: ET1612-12
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | SD2002 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 21 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ARF1aa 61-110 / 181. |
| Positive control: | NIH/3T3 cell lysates, human colon carcinoma tissue, human breast carcinoma tissue, Hela. |
| Subcellular location: | Golgi apparatus, Cytoplasm, Cell junction , Membrane. |
| Recommended Dilutions:
WB FC IHC-P |
1:1,000-1:2,000 1:50-1:100 1:100 |
| Uniprot #: | SwissProt: P84077 Human | P84078 Mouse | P84079 Rat |
| Alternative names: | ADP Ribosylation Factor 1 ADP-ribosylation factor 1 ARF 1 ARF1 ARF1_HUMAN |
Images
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Fig1:
Western blot analysis of ARF1 on different lysates with Rabbit anti-ARF1 antibody (ET1612-12) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ARF1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-12) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of ARF1 on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-12, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-ARF1 antibody (ET1612-12) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-12) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-ARF1 antibody (ET1612-12) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-12) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of ARF1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-12, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.