RelB Recombinant Rabbit Monoclonal Antibody [SD07-39]
cat.: ET1612-18
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SD07-39 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human RelB aa 1-50 / 579. |
| Positive control: | Raji cell lysate, Daudi cell lysate, human tonsil tissue. |
| Subcellular location: | Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IP |
1:1,000 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q01201 Human |
| Alternative names: | I REL I-Rel IREL Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 relB RELB_HUMAN Reticuloendotheliosis viral oncogene homolog B Transcription factor Rel B Transcription factor RelB v rel avian reticuloendotheliosis viral oncogene homolog B v rel reticuloendotheliosis viral oncogene homolog B |
Images
|
Fig1:
Western blot analysis of RelB on different lysates with Rabbit anti-RelB antibody (ET1612-18) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-18) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-RelB antibody (ET1612-18) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-18) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.