Prostate Specific Antigen Recombinant Rabbit Monoclonal Antibody [SD07-45]
cat.: ET1612-19
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SD07-45 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 34 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Prostate Specific Antigen aa 198-261/261. |
| Positive control: | PC-3M, human prostate tissue, human prostate carcinoma tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P07288 Human |
| Alternative names: | antigen, prostate-specific APS Gamma seminoprotein Gamma-seminoprotein hK3 Kallikrein 3 Kallikrein related peptidase 3 Kallikrein-3 KLK 3 KLK2A1 Klk3 KLK3_HUMAN P-30 antigen P30 antigen Prostate-specific antigen Psa Semenogelase Seminin |
Images
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Fig1: ICC staining of Prostate Specific Antigen in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-Prostate Specific Antigen antibody (ET1612-19) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-19) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-Prostate Specific Antigen antibody (ET1612-19) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-19) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.