Anti-STAT1 alpha antibody [SD20-75]
cat.: ET1612-22
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC/IF, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: SD20-75
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 87 kDa
Isotype: IgG
Immunogen: recombinant protein
Positive control: SKOV-3, MCF-7, Hela, 293, human cervix uteri tissue.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC

1:1,000-1:5,000
1:100-1:500
1:50-1:200
1:50-1:100
Uniprot #: P42224(Human)
Alternative names: CANDF7 antibody DKFZp686B04100 antibody ISGF 3 antibody ISGF3 antibody OTTHUMP00000163552 antibody OTTHUMP00000165046 antibody OTTHUMP00000165047 antibody OTTHUMP00000205845 antibody Signal transducer and activator of transcription 1 antibody Signal transducer and activator of transcription 1, 91kDa antibody Signal transducer and activator of transcription 1-alpha/beta antibody Stat1 antibody STAT1_HUMAN antibody STAT91 antibody Transcription factor ISGF-3 components p91/p84 antibody
Images
ET1612-22_1.jpg Fig1: Western blot analysis of STAT1 on different lysates using anti-STAT1 alpha antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293
ET1612-22_2.jpg Fig2: ICC staining STAT1 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1612-22_3.jpg Fig3: ICC staining STAT1 alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1612-22_4.jpg Fig4: ICC staining STAT1 alpha in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1612-22_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human cervix uteri tissue using anti-STAT1 alpha antibody. Counter stained with hematoxylin.
ET1612-22_6.jpg Fig6: Flow cytometric analysis of MCF-7 cells with STAT1 alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.