Anti-Cdk4 antibody [SD205-1]
cat.: ET1612-23
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IP
Clonality: Monoclonal
Clone number: SD205-1
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 34 kDa
Isotype: IgG
Immunogen: Recombinant protein within mouse Cdk4 aa 180-303.
Positive control: Hela cell lysate, MCF-7 cell lysate, K562 cell lysate, Hela, AGS, NIH/3T3.
Subcellular location: Cytoplasm, Nucleus, Membrane.
Recommended Dilutions:
  WB
  ICC/IF
  IP

1:1,000-1:2,000
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: P11802 Human | P30285 Mouse | P35426 Rat
Alternative names: Cdk 4 antibody cdk4 antibody CDK4 protein antibody CDK4_HUMAN antibody Cell division kinase 4 antibody Cell division protein kinase 4 antibody CMM 3 antibody CMM3 antibody Crk3 antibody Cyclin dependent kinase 4 antibody Cyclin-dependent kinase 4 antibody Melanoma cutaneous malignant 3 antibody MGC14458 antibody p34 cdk4 antibody PSK J3 antibody PSK-J3 antibody
Images
ET1612-23_1.jpg Fig1: Western blot analysis of Cdk4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: MCF-7 cell lysate
Lane 2: K562 cell lysate
ET1612-23_2.jpg Fig2: ICC staining of Cdk4 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-23_3.jpg Fig3: ICC staining of Cdk4 in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-23_4.jpg Fig4: ICC staining of Cdk4 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.