Anti-PDPK1 antibody [SD204-9]
cat.: ET1612-27
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P
Clonality: Monoclonal
Clone number: SD204-9
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 63 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human PDPK1 aa 1-50.
Positive control: 293T cell lysate, PC-12 cell lysate, HL-60 cell lysate, Hela, MCF-7, SW480, human breast carcinoma tissue, mouse kidney tissue, human colon carcinoma tissue, human kidney tissue.
Subcellular location: Cell membrane, Nucleus, Cytoplasm, focal adhesion.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P

1:500-1:2,000
1:100-1:500
1:50-1:200
Uniprot #: SwissProt: O15530 Human | Q9Z2A0 Mouse | O55173 Rat
Alternative names: 3 phosphoinositide dependent protein kinase 1 antibody 3-phosphoinositide-dependent protein kinase 1 antibody hPDK 1 antibody hPDK1 antibody MGC20087 antibody MGC35290 antibody OTTHUMP00000159109 antibody OTTHUMP00000159110 antibody OTTHUMP00000174525 antibody PDK1 antibody Pdpk1 antibody PDPK1_HUMAN antibody PDPK2 antibody PDPK2P antibody PkB kinase antibody PkB kinase like gene 1 antibody PkB like 1 antibody PRO0461 antibody Protein kinase antibody
Images
ET1612-27_1.jpg Fig1: Western blot analysis of PDPK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: SK-Br-3 cell lysate
ET1612-27_2.jpg Fig2: ICC staining of PDPK1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-27, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-27_3.jpg Fig3: ICC staining of PDPK1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-27, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-27_4.jpg Fig4: ICC staining of PDPK1 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-27, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-27_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PDPK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-27_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PDPK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-27_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PDPK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-27_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDPK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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