pro Caspase-7 Recombinant Rabbit Monoclonal Antibody [SD20-57]
cat.: ET1612-28
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SD20-57 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Caspase 7 aa 1-50 / 303. |
| Positive control: | Hela, HepG2, human liver tissue, human kidney tissue,human lung lysates. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P55210 Human |
| Alternative names: | Caspase7 apoptosis-related cysteine peptidase Apoptotic protease Mch-3 CASP-7 CASP7 CASP7_HUMAN Caspase-7 subunit p11 Caspase-7 Caspase 7 Caspase7 CMH-1 ICE-LAP3 ICE-like apoptotic protease 3 |
Images
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Fig1:
Western blot analysis of pro Caspase-7 on human lung lysates with Rabbit anti-pro Caspase-7 antibody (ET1612-28) at 1/1000 dilution. Lysates at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 35 kDa Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-28) at 1/1000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining pro Caspase 7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining pro Caspase 7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-pro Caspase 7 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-pro Caspase 7 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.